Laboratory tests included bacterial culture, nucleic acid testing, serological testing, and whole genome sequencing of Bordetella pertussis.

(1) Specimen collection. Nasopharyngeal swabs were collected preferentially by bacterial culture and nucleic acid detection, followed by nasopharyngeal aspirate. Serological tests were performed to collect single or double serum (acute and convalescent).

(2) Specimen testing and transportation. After collecting specimens, medical institutions should be inoculated into the medium as soon as possible, and can also be inoculated into the medium after increased culture to improve the positive rate of culture.

The remaining samples after inoculation can be used for nucleic acid detection. If you need to improve the sensitivity of nucleic acid detection, it is recommended to collect specimens separately. The serological test uses PT-igg antibodies in two or one serum,

and should consider the possible influence of infants and young children, vaccination with pertussis components within 1 year, or recent infection resulting in increased PT-igg antibodies.

If the specimen cannot be tested in time after collection, it should be stored in a standardized manner and transported to a professional laboratory. Bacterial genome sequencing was carried out in accordance with the genome sequencing operation specifications of the National Pathogen Identification Network, and the sequencing results were uploaded to the National Pathogen Identification Network in a timely manner.

Give full play to the role of new media and traditional media such as radio, television and newspapers, widely publicize popular science knowledge of pertussis prevention and control,

enhance public awareness of protection, develop good hygiene habits such as wearing masks, washing hands frequently, and maintaining cough etiquette, and be the first responsible person for their own health.

Strengthen the publicity and education of the public on vaccination knowledge, and encourage guardians to ensure that school-age children receive the vaccine containing pertussis components as early as possible and throughout according to law.

Strengthen maternity centers, nursery institutions, primary and secondary schools, child welfare agencies and children
Popularizing knowledge and science-based epidemic prevention

In the Spring Festival of the Year of Boxer in 2020, the new coronavirus epidemic did not stop the pace of transmission because of the arrival of the festival. The real-time situation of the epidemic has become a daily must-read for every Chinese.

But at the same time, all kinds of rumors have emerged, some people say that salt water gargling can prevent the virus, some people say that smoking and drinking can prevent the virus, and some people say that lighting firecrackers is conducive to killing the virus.

How to strengthen the public's awareness and understanding of the novel coronavirus pneumonia and enhance the public's confidence in the fight against the novel coronavirus pneumonia, scientific, effective and accurate transmission of relevant knowledge has become a top priority.

In the form of a comic book, the manual introduces basic medical knowledge, personal and family protection, and protection in public places in three parts in a popular language style. Ensuring the unity of science and accessibility is a major feature of the manual.

Pay attention to the basic knowledge, select authoritative experts to write, to ensure the scientific standard of the content; The protective measures for individuals and families have been carefully sorted out,

and the prevention and control details including how to wash hands, how to put on and take off masks have been clearly designed from the specific scenes and specific environments that individuals and families often face.

Unlike traditional popular science books filled with a large number of scientific and technological terms and terminology, the manual language is popular, concise and accurate, and strives to narrow the distance with readers through the most understandable words, and strives to understand at a glance.

Different from the traditional popular science books, the protection content explanation part of the manual explains the concept clearly, and describes the scope of application by suggesting the main points of specific protection actions,

which is very easy to accept. Unlike the traditional popular science books, which are not very interesting, the manual introduces the well-known network painters through cartoons, which is very close to the people's cognitive habits, and vividly, lively and accurate introduces a variety of protective equipment and its use.

Starting with the needs of the audience and going straight to the core issue of how to protect is another feature of this manual. On the one hand, it solved the problems that the people were most concerned about by Q&A,

summarized 40 of the most common questions that the people faced with the novel coronavirus pneumonia, and gave standardized answers one by one. It not only analyzes the most common basic problems such as how to understand the new coronavirus and what the coronavirus is afraid of,

but also considers solutions such as precautions for home medical observation and how to help family members accept isolation and other specific scenarios. It not only discusses the basic protection of individual family,

but also explains how to protect the public environment. At the same time, this way of highlighting application scenarios and specific protection methods also further improves the applicability of the manual.